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Integrin beta1-mediated adhesion of human B lymphoblasts to lymph node stroma.
The biological importance of adhesion between lymphocytes and stromal cells in lymphoid tissues is not known. Using an in vitro adhesion assay, we found that the beta1 integrin, which can bind a variety of extracellular matrix proteins, mediated adhesion of human B lymphoblasts to stromal cells in lymph nodes. Strong adhesion was observed between tonsil stroma and tonsil B lymphoblasts as compared with the binding of lymphoblasts to other types of stromal cells or to lymphocytes. Adhesion of lymphoblasts to tonsil stroma was calcium independent and required either immobilized or soluble fibronectin. It was inhibited by anti-beta1 integrin monoclonal antibodies and by a peptide inhibitor of fibronectin binding. Adhesion of lymphoblasts to tonsil stroma was also inhibited by RGD-containing peptides or antibodies to alpha3 or alpha5 integrin subunits. In contrast, neither integrin-alpha1, alpha2, alpha4, alpha6, alphaV, beta2, or beta7 subunits nor their ligands were able to mediate adhesion of lymphoblasts to tonsil stroma. Furthermore, binding of antibodies to the extracellular domains of alpha3 or alpha5 integrin subunits inhibited adhesion of lymphoblasts to both lymph node stroma and lymphocyte surface, and adhesion of lymphoblasts to tissue sections of lymph node was not affected by antibodies to alpha3 or alpha5. These results indicate that lymphocytes adhere to the stroma of lymph nodes in a beta1-mediated manner, and suggest that beta1 integrin may be important in both the localization and activation of lymphoid cells.Are the lungs the culprit in end-stage renal disease?
End-stage renal disease (ESRD) is a chronic condition associated with significant morbidity
What’s New In?
New commands for the Drafting Room visualization tool enable you to adjust and manipulate graphics and text, apply edit, freeze, and unfreeze changes.
Draw, Fill, and Stroke:
Shapes can now be filled, outlined, or filled with a gradient that changes color along the path. (video: 1:22 min.)
Edit and Repair:
Faster and easier ways to adjust color, line width, and style. Add text and an image to a shape to create a new one. (video: 1:15 min.)
Gather options in groups of nodes for enhanced performance in small, complex drawings. (video: 1:15 min.)
Point to Set:
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Edit and Repair:
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The boundary snap tool now snaps to multiple snap points. (video: 1:16 min.)
The latest release of the free Fusion 360 design app is available. (video: 1:16 min.)
Create and edit animation sequences using the animation tool. (video: 1:14 min.)
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Mac OS X 10.9 or later
Intel Core i5 or equivalent
NVIDIA GeForce GTX 970 or equivalent
4K display (3840×2160)
Intel Core i7 or equivalent
NVIDIA GeForce GTX 1060 or equivalent
8K display (7680×4320)
About this game
Upcoming: Upgrade to 1.3
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